While in the crystal composition of your D273N mutant of NagA, the facet chain of His-143 is 3.0 Å clear of the phosphonate oxygen on the mimic in the tetrahedral intermediate (21). This end result indicates that this team could facilitate the activation with the carbonyl team in the substrate at the side of an interaction While using the metallic ion sure throughout the Energetic internet site.
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a worth to the ionization on the guanidino team is expected to exceed 12. Nonetheless, in NagA from E. coli
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In Balinese tradition, nagas are frequently depicted battling garuḍas. Intricately carved nagas are discovered as stairs railings in bridges or stairs, for example These present in Balinese temples, Ubud monkey forest, and Taman Sari in Yogyakarta.
Residues Q59 and N61 ended up mutated with each other to a pair of histidine residues to build an HxH motif that may be analogous to that found in NagA from B. subtilis
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